SCIENTIFIC PUBLICATIONS & STANDARD OPERATING PROCEDURES
Detection of PD-L1 and PD-L2 on Circulating Tumor Cells (CTCs) Using Chipcytometry
ChipCytometry identifies the presence of uncommon B cell subset in inflamed tonsils associated with autoimmunity
Comparison between ChipCytometry and Flow Cytometry for Biomarkers and Immunophenotyping Applications
Validation of Treg, Th17 and Plasma Cell Assays
Comparison of Human Whole Blood Immunophenotyping by ChipCytometry and Flow Cytometry
High-Parameter Profiling of Psoriatic Tissue
Cross-technology Comparison of Chipcytometry vs. Flow Cytometry for the Measurement of T Cell Phenotypic and Functional Markers
Cellular analysis of induced sputum by Chipcytometry
In Situ Detection of Mesenchymal Stromal Cells in Human Placenta by Chipcytometry
Cytegeist: A framework for biological data analysis
MAIT cells in media/nal lymph node TB
Imaging cytometry: the advantages of hybrid technology in support of drug discovery
A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based Chipcytometry
IMAGE CYTOMETRY: A NEW METHOD FOR DEEP PHENOTYPING OF RARE CELLS IN THE BLOOD OF ONCOLOGY PATIENTS
SUPPORTING CLINICAL TRIALS – THE IMPACT OF SAMPLE PREPARATION ON IMMUNOLOGY DATA
A DIAGNOSTIC TOOL FOR RED BLOOD CELL DISORDERS – CHIPCYTOMETRY AS A NEW RBC ANALYSIS PLATFORM
Chipcytometry: Long-term Sample Storage and Re-Interrogation in Clinical Trial Support
PUBLICATIONS DESCRIBING ASPECTS OF THE TECHNOLOGY
PUBLICATIONS USING CHIPCYTOMETRY
Activation and In Vivo Evolution of the MAIT Cell Transcriptome in Mice and Humans Reveals Tissue Repair Functionality
In this Cell paper, the authors define a basic transcriptome of an activated MAIT cell in mice and humans which is most similar to iNKT cells during acute infection and more closely resemble yd T cells after resolution of infection.
Long-term in vivo microscopy of CAR T cell dynamics during eradication of CNS lymphoma in mice
In this PNAS paper, the successful attack of a CNS lymphoma by CAR T cells was demonstrated in a mouse model.
A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based ChipCytometry
In this study, David Skibinski and colleagues from Merck Singapore developed a method that applies ChipCytometry for the detection and phenotyping of circulating tumor cells in blood.
Gain-of-function STAT1 mutations are associated with intracranial aneurysms
Dadak M, Jacobs R, Skuljec J, Jirmo AC, Yildiz Ö, Donnerstag F, Baerlecken NT, Schmidt RE, Lanfermann H, Skripuletz T, Schwenkenbecher P, Kleinschnitz C, Tumani H, Stangel M, Pul R. Clin Immunol. 2017.
Monocyte/macrophage lineage commitment and distribution are affected by the lack of regulatory T cells in scurfy mice
Skuljec J, Cabanski M, Surdziel E, Lachmann N, Brennig S, Pul R, Jirmo AC, Habener A, Visic J, Daluege K, Hennig C, Moritz T, Happle C, Hansen G. Eur J Immunol. 2016.
a-NAC-specific autoreactive CD8+ T cells in atopic dermatitis are of an effector-memory 2 type and secrete IL-4 and IFN-y
Antigen-specific T cells were detected and phenotyped using the rare cell detection feature of ChipCytometry and MHC class I specific tetramers on living cells immobilized inside XXL chips.
Roesner LM, Heratizadeh A, Wieschowski S, Mittermann I, Valenta R, Eiz-Vesper B, Hennig C, Hansen G, Falk CS, Werfel T. Journal of Immunology. 2016.
Der p1 and Der p2-specific T cells display a Th2, Th17, and Th2/Th17 phenotype in atopic dermatitis
Roesner LM, Heratizadeh A,Begemann G,Kienlin P,Hradetzky S,Niebuhr M,Eiz-Vesper B,Hennig C,Hansen G,Baron-Bodo V,Moingeon P,Werfel T. Journal of Investigative Dermatology. 2015.
Pulmonary transplantation of macrophage progenitors as effective and long-lasting therapy for hereditary pulmonary alveolar proteinosis
In this excellent study, both single cell and tissue cytometry options of ChipCytometry were applied to implement a high-content in-depth analysis of transplanted cells in a humanized mouse model.
Happle C, Lachmann N, Skuljec J, Wetzke M, Ackermann M, Brennig S, Mucci A, Chari AJ, Groos S, Mirenska A, Hennig C, Rodt T, Bankstahl JP, Schwerk N, Moritz T, Hansen G. Science Translational Medicine. 2014.
Successful treatment of autoimmune and lymphoproliferative complications of patients with intrinsic B-cell immunodeficiencies with Rituximab
Hennig C, Baumann U, Ilginus C, Horneff G, Foell J, Hansen G. Br J Haematol. 2010.
Identification and quantification of basophils in the airways of asthmatics following segmental allergen challenge
Dijkstra D, Hennig C, Hansen G, Biller H, Krug N, Hohlfeld JM. Cytometry A. 2014.
Allergy prevention starts before conception: maternofetal transfer of tolerance protects against the development of asthma
ChipCytometry was used to analyze the antigen-specific immune response in the offspring of mice tolerized to a specific allergen. Surprisingly, interferon-γ producing T cells were found to be induced in the offspring due to diaplacentar immune complex transfer.
Polte T, Hennig C, Hansen G.J Allergy Clin Immunol. 2008.
CD3+CD20+ T cells in peripheral blood: Proper flow cytometry is a prerequisite of exact phenotyping
ChipCytometry was applied as a supportive technology to confirm flow cytometry results that demonstrated the existence of T cells expressing CD20.
Wilk E, Witte T, Marquardt N, Hennig C, Hansen G, Schmidt RE, Jacobs R. Arthritis Rheum. 2010.
Lung function and inflammation during murine Pseudomonas aeruginosa airway infection
Woelbeling F, Munder A, Kerber-Momot T, Neumann D, Hennig C, Hansen G, Tuemmler B, Baumann U. Immunobiology. 2011.